These additives are particularly suitable for situations where high protein concentration and long-term stability are required, including solution-state studies of isotopically labeled proteins by NMR. Specific protein-protein and protein-RNA interactions are not adversely affected by the presence of these amino acids. Several methods for protein precipitation are described in. (11469 g, 15 min, 4 ☌) and the pellet obtained was solubilized in sodium acetate buffer (0.1 M, pH 4. protein pellet is re-dissolved in a buffer that is compatible with the downstream application. These amino acids are effective in preventing protein aggregation and precipitation, and they dramatically increase the long-term stability of the sample additionally, they protect protein samples from proteolytic degradation. Biological protein precipitation: A green process for the extraction of cucumisin from melon (Cucumis melo L. We demonstrate here that simultaneous addition of charged amino acids L-Arg and L-Glu at 50 mM to the buffer can dramatically increase the maximum achievable concentration of soluble protein (up to 8.7 times). Increasing a protein concentration in solution to the required level, without causing aggregation and precipitation is often a challenging but important task, especially in the field of structural biology as little as 20% of nonmembrane proteins have been found to be suitable candidates for structural studies predominantly due to poor protein solubility. Protein buffer is 50 mM tris pH 7.5, 500mM NaCl, 5 glycerol and 10mM beta ME and pI is 6.0.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |